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Unlike GLP-1RAs, particularly dulaglutide (254), SGLT-2 inhibitors have not demonstrated cardiovascular benefits in primary prevention among individuals with multiple cardiovascular risk factors (261)

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The construct was expressed in Spodoptera frugiperda ( Sf 9) insect cells (Invitrogen) with Bac-to-Bac Baculovirus system (Invitrogen) and cells were infected at a density of 2 10 6 cells per mL and collected 48 h after infection

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